According to the shift in sheet resistance and different morphologies observed by atomic force microscopy, it can be concluded that for Au nanolayer deposited under 300°C, the insulating layer between gold nanoclusters
causes shift of the MI-503 mw surface plasmon resonance peak, as was observed e.g. in  for graphene and Au nanoparticles. On the basis of the achieved results, it can be concluded that electrically buy VRT752271 continuous metal nanolayers with very low surface roughness can be prepared by evaporation on the substrate at elevated temperature. These structures also exhibit peaks of plasmon resonance up to Au thickness of 10 nm. The combination of surface plasmon resonance together with
low surface roughness may find applications in the construction of biosensors for the detection of mycotoxins . On the contrary, structures with different densities of gold nanoclusters prepared by the technique of evaporation at RT or consequently annealed can be of a great contribution for the construction of biosensors and DNA detection . CYT387 Depth analysis The difference in surface metal distribution of evaporated structures under RT and evaporated onto substrate heated to 300°C is evaluated in Figure 7. The difference in the behavior of surface nanostructures in area on electrical discontinuity and continuity can be clearly seen. The electrically discontinuous layer exhibits significantly higher gold concentration when deposited on non-heated substrate. The heat treatment seems to be a positive promoter of surface diffusion (and nanocluster growth), mostly in the early stages of gold layer growth. This difference, thus, seems to affect the surface gold concentration; the higher the surface concentration, the more homogeneous the layer is. On the contrary, for higher gold thicknesses, when the layer is already electrically
continuous, this difference is reversed. The influence of heated substrate causes the decrease of isolated nanocluster formation and thus positively ifenprodil influences its homogeneity. The isolated nanostructure, being less pronounced, increases the absolute gold concentration. Figure 7 RBS spectra of gold structures. RBS spectra of gold structures evaporated on glass with room temperature and Au nanostructures evaporated on glass heated to 300°C (300°C). Conclusions The different surface properties of thermally annealed gold nanostructures in comparison to those evaporated onto heated substrate has been described. The heating of glass during the evaporation results in dramatic changes of the surface morphology and roughness. The substrate heating leads to the decrease of surface roughness for higher Au thickness, the electrical properties being also strongly influenced, the structure being more homogeneous.