The mean value of the yield load was in the PTH group higher as compared to the E and C and sham group, but these changes were Vadimezan concentration statistically not significant (Table 1). Table 1 The results from comparative bioassay: body weight, biomechanical test, histomorphometry, and serum
analysis Sham OVX Estradiol benzoate Parathyroid hormone AZD5582 nmr Mean STD Mean STD Mean STD Mean STD Body weight (g) 275.6a 14.31 342.2 19.91 280.3a 12.05 324.9b,c 19.38 Serum analysis Osteocalcin (ng/ml) 2a 2.0 17.78 5.64 5.347a 1.79 45.46a,b,c 5.22 Crosslaps (ng/ml) 4.04a 0.25 33.83 8.37 46.86 34.25 45.66b 19.56 Biomechanical this website test Maximum load (N) 192.1a 20.49 166.03 38.36 182.92b 13.83 225.25a,b,c 46.55 Yield load (N) 120.2 16.48 111.57 31.33 113.14 10.04 132.00 18.69 Stiffness (N/mm) 267.0a 26.10 235.56 40.82 237.15b 45.40 314.87a,b,c 72.05 Histomorphometry N.Nd/mm2 48.54a 5.439 34.35 6.97 40.66b 6.24 41.32a 4.36 Tb.Ar (%) 77.25a 10.73
57.18 13.62 61.04b 8.27 75.65ac 9.02 Tb.Wi (mcm) 8.5a 1.38 7.62 0.95 7.53b 1.25 9.80abc 1.27 B.Dm (mcm) 3,154 135.9 3,137 280.6 3,140 161.1 3,151 124.1 Ma.Dm (mcm) 1,814 67.78 1,838 221.4 1,792 123.4 1,615a,b,c 132.5 B.Dm/Ma.Dm 1.740 0.063 1.716 0.08 1.749 0.069 1.938a,b,c 0.069 Tb.Ar ratio of trabecular
area, N.Nd/mm 2 connectivity, Tb.Wi trabecular thickness, B.Dm bone diameter, Ma.Dm marrow diameter aSham/E/PTH vs. OVX (p < 0.05) bE/PTH vs. sham (p < 0.05) cPTH vs. E (p < 0.05) Histomorphometric changes in the proximal femur after administration Thiamet G of estradiol and parathyroid hormone The results of the histomorphometric analysis and micro-architectural parameters are summarized in Table 1. The results of Tb.Ar, N.Nd/mm2, and Tb.Wi were significantly higher in the PTH group (Tb.Ar = 75.65%, N.Nd/mm2 = 41.32, Tb.Wi = 9.80 µm) in comparison to the C group (Tb.Ar = 57.18%, N.Nd/mm2 = 34.35, Tb.Wi = 7.62 µm). We found a significantly higher value for the PTH compared to E groups concerning the Tb.Ar. Although the mean values of Tb.Ar, N.Nd/mm2, and Tb.Wi were higher in the E-treated rats (Tb.Ar = 61.04%, N.Nd/mm2 = 40.66, Tb.Wi = 7.53) than in the C, these differences were statistically not significant. The Tb.Wi in the PTH rats was significantly higher compared to the sham animals (Tb.Wi = 8.5 µm; Fig. 5). Fig. 5 Microradiographs of the proximal femur of rat show the high content of both cortical and trabecular surfaces (after breaking test).