The extract has been used as a pink and purple food coloring agent as well as a spice to give a sore-sweet taste. Its syrup is consumed as a soft drink during summer. In addition to food usage, it has also been used as a cosmetic ingredient, as well as a traditional medicine for treatment of inflammation and other disorders. In spite of its wide economical importance, a rapid and efficient method for its identification and quantification is lacking. In addition garcinol is always present along with another compound isogarcinol in kokum fruit. 1, 2, 3, 4, 5, 6, 7 and 8 Hence

a new HPLC 9, 10 and 11 analysis method for simultaneous analysis of garcinol and isogarcinol was developed. The aim of the A-1210477 purchase present study was to develop a rapid, economical, precise and accurate reversed-phase HPLC method with wide linear range and a good sensitivity for Akt inhibitor the determination of garcinol and isogarcinol. In this study, HPLC instrumentation with UV detection, which is readily available in most analytical and pharmaceutical laboratories, was used. The analytical method was

validated as per current International Conference on Harmonization (ICH) guidelines.12 Acetonitrile (HPLC grade, MERCK), Water (HPLC grade, Thomas Baker) and orthophosphoric acid (AR grade), di-n-butyl phthlate (AR grade), G. indica fruit rind, garcinol and isogarcinol are procured from local analytical laboratories. HPLC is a chromatographic technique MTMR9 used to separate a mixture of compounds in analytical chemistry and biochemistry with the purpose of identifying, quantifying & purifying the individual components of the mixture. The HPLC system consisted of Agilent 1200 and equipped with quaternary pump G1331A connected with G1314B variable wavelength detector, G1316A thermostatted column compartment, G1329A ALS autosampler. The data acquisition was performed by

Agilent Chemstation software. The chromatographic separation was achieved on Zorbax SB C-8 (150 mm × 4.6 mm i.d., 3.5 μm) column. The elution was isocratic with mobile phase of 0.1% orthophosphoric acid in water and acetonitrile (20:80, v/v). The flow rate was 1.0 mL/min and yielded a backpressure of about 57 bar. The column temperature was maintained at 40 °C, the detection was monitored at a wavelength of 215 nm and injection volume was 5 μL. HPLC is suitable for simultaneous separation of garcinol and isogarcinol with di-n-butyl phthlate as internal standard. The standard stock and sample solutions were prepared with di-n-butyl phthlate in acetonitrile to give the final concentration of 250 μg/mL concentration of both garcinol and isogarcinol. The working standard solution of garcinol and isogarcinol were prepared by taking suitable dilutions. For the analysis of garcinol and isogarcinol in G. indica 200 g of fruit rind was powdered and extracted in methanol.