The animal pathway was continued with a short 30 mm piece of large tubing followed by a 180 mm length of small

tube that would be used to cannulate the animal. To allow access from outside the magnet, an ∼800 mm length of small tubing was attached upstream of the two-way cannula with a 21 gauge luer Quizartinib hub glued at one end and with the other end potted into a male–male Luer adapter (Cole-Parmer) using dental cement, see Fig. 3. Fluid flow pathway was guided using a custom-made pinch valve and a normally closed diaphragm valve (PMDP-2R-M6G, Takasago, Nagoya, Japan), both actuated by pneumatic pressure and controlled by an Arduino microcontroller. A custom made pinch valve chassis was machined from polycarbonate (PC) with a 10 mm syringe/plunger that actuated a PC cylinder that acted on the Tygon tube, see Fig. 3. When air pressure was applied either one or both

valves could be opened or closed. Air pressure was supplied by a custom made pneumatic control box with independent control valves that could be turned on by a 5 V input provided by the Arduino microcontroller. Once the diverter cannula had been surgically inserted into the animal and the animal transferred to the magnet, the animal pathway line was inserted into the pinch valve (at the Tygon position) and held in place by a plastic gate. selleck inhibitor The waste line of the fluid path was connected to the diaphragm valve and the 3-way stopcock opened to provide a continuous fluid outlet path to waste outside Docetaxel ic50 of the magnet. On injection, the Arduino microcontroller was programed to open the waste line valve and close the rat line valve and pump. Typically 0.6–0.8 ml of liquid went

to waste. The fluid path was then switched to the rat by opening the rat line valve and closing the waste line and the desired injection volume was delivered to the rat. During idle mode, the rat side valve was left open to prevent damage to the Tygon tube. The injector pump system was controlled through a computer serial link to an Arduino Uno R3 microcontroller (Arduino.cc). The microcontroller controlled the stepper motor via custom made stepper motor driver electronics. The flow diverter and air stirrer were operated via a custom made pneumatic control box which provided air pressure (pre-set at 40–60 PSI) in response to 5 V input signals. Trigger of the injection sequence was started in response to a 5 V signal (greater than 10 ms duration to eliminate false triggering) from either the HyperSense polarizer or scanner console to the microcontroller. Once the injection system had been placed in trigger standby, the pH in the RV was reported every 30 s for up to 2 min 10 s (a duration chosen to be ∼20 s shorter than the polarizer’s dissolution solvent heating preparation time).