Ponds, with a working volume of 14 L, were used per treatment. The fish were left to acclimatize for 3 days of and w was During the eight days after the playback compatibility T of each pair of t Ability assessed by the possible observation of the behavior of the normal playback, the number of eggs and Lebensf Eggs. Fish parts from the same batch, which was held under the same conditions were used for the couple, the spawn did not want to replace. After assessing the compatibility of reproduction, bicalutamide was tested in the test system and more than 28 days are, onreproductive effects were evaluated. Batches of eggs were collected from ponds and assessed for the number of fertilized and unfertilized egg batch. A minimum of two phone start-up Estimates for hatching were each of 50 embryos produced by breeding pairs. If an RAF Signaling Pathway insufficient number of batches were prepared from 50 embryos, the analyzes were performed on smaller lots. Selected COOLED embryos were transferred into two cups of incubation, 25 embryos in each cup. They were either exposed to 9.5 L working volume descent tanks for each treatment were mechanically up and down in the water Column, twice per minute. The number of live and dead embryos were t Resembled recorded and discarded dead embryos. Once the eruption was completely larvae were to end human. The embryos from the same spawning days between a minimum of two females were scored per treatment and collected randomly assigned to four cups of incubation. After hatching, they were in a container Container per treatment of Minderj YEAR OLD, a working volume of 13 L. Approximately 30 DPH had dismissed all surviving fish in each treatment group were transferred to 45 L working volume tanks adults. If the fish tested DPH were about 84 meters it was Possible, gender, the majority of the eye and breeding groups in each of the two Laichbeh Assigned ltering by repeated treatment. Two
spawning substrates were provided for each spawning tanks. The remaining fish were obtained in the tanks of adult until the end of the study. In the lter Laichbeh, the presence or absence of embryos was recorded for 10 days, and the number of embryos protected, screened using the following four categories of ratings: 0 0 1 50 2 51 200 and 3201 embryos per tank. At the end of the study, all fish in all tanks were get under the provisions of the Home Office Tet, followed in buffered MS222, of the destruction Tion of the brain. Weight was obtained immediately after a full tank of termination and the fish were measured. SSC were determined and the carcass of the fish in Bouin, s fixed for histological and microscopic examination, as described below. 2.5. The histological analysis of whole fish fixed in Bouin, were the L S from solution in the pelvic fin cross-sections in the region of the gonads to cut. Three sections were fish. The tissues were embedded in paraffin and sectioned at about 2 4 m thick, mounted on a Objekttr hunter and with H Matoxylin and eosin. These sections were microscopically to determine if m Possible, fish sex and stage of development of the testes or the Eierst CKE Investigated. In short, steps one through five, as testicular sperm cells with sex to describe Of:. Rest of the germs cells, spermatogonia, spermatocytes, spermatids and sperm Given some of the Samenkan Lchens