Ter oral AZA administration.8 Once inside the cell, 6-MP to its active metabolite, thioguanine nucleotide metabolism, the sixth 6 NSGCT, due to their structural similarity With the guanine base pair endogenous, incorporated into the DNA of leukocytes and to train breakage.9, 10 a 6 TGN, 6 thioguanine triphosphate, has been shown that apoptosis increased Hen in the peripheral blood, both on the blade and gut propria T cells by inhibiting Rac1, inhibits apoptosis glutamyltranspeptidase. June 11 MP was also shown to induce apoptosis in lymphocytes activated splenic B lipopolysaccharide. 12 nucleobase physiological nucleosides and their analogues are hydrophilic and are thought to ben transport proteins Term to cross the cell membrane physiologically sufficient rates.13, is known 14 about the specific nucleobase transport and therefore the method transport 6 MP still poorly understood. Nucleobase transport of nucleoside transporters has been described.15 proteasome inhibitors several absorption Tr Can be expressed in human tissues like k To contribute to the intracellular Re accumulation of 6 MP, including normal Quilibrierenden nucleoside transporter 1, 2, 3, 4 and nucleoside transporter concentration of 1, 2, 3.16 18 CNT 1 and 2 from sodium as cation Co transported, can cooperate CNT 3 sodium transport as well as the lithium or hydrogen ions k. The Tr Hunters are not balancing sodium dependent.19 variations you can in the intracellular Re accumulation of 6 MP lead to differences in the reqs Susceptibility to cytotoxicity Tons of drugs. Thus we are intracellular Re accumulation of 6 MP and 6 MP reqs Susceptibility evaluated for cytotoxicity t in a panel of transformed human lymphocytes derived from controlled And IBD patients.
The expression of 15 potential nucleoside transporters in these cell lines was intracellularly in an attempt to the carrier hunter with for correlation of the Re accumulation 6 MP identify investigated. Materials and Methods Cell lines under a Council Johns Hopkins Medicine Institutional Review were approved protocol, peripheral mononuclear Re cells isolated from 10 patients with IBD. Einverst Ndniserkl Ments were obtained prior to study entry. Thiopurine methyltransferase genotype or enzyme activity, S t was not available for most patients. Cells with 5 ml of blood Epstein-Barr virus-transformed according to standard protocol at Johns Hopkins were Center.20 Cell, 21 complements transformed lymphocytes in suspension in RPMI 1640 erg With 10% fetal K Calf serum and 0.5% penicillin / streptomycin in an atmosphere re of carbon dioxide in oxygen of 5% to 37% to 95 Chemicals Unless otherwise stated, all the chemicals that used in this study from Sigma Erlotinib 183319-69-9 Aldrich. Transportation account cell assay were first Highest using an H Mozytometers determined. A standard curve was then nm for each cell line by correlation of the number of cells with an optical density at 600 created on a Beckman DU 640 spectrophotometer. After Zellz Hlung was performed by measuring the OD at 600 nm. The determination of the traffic was performed in 150 ml volume. 14C radioactivity was 6 MP in water with a mol / l caustic soda At a concentration of 3.8 mg / ml of gel St. These L Solution was then added to each aliquot of cells to obtain a drug concentration of 0.05 mg / ml. In the previous study with human T-cell culture with AZA Conc.