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B, Van Roy N, De Paepe A, et al.: Accurate normalization selleck compound of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002, 3:research0034.PubMedCrossRef N-acetylglucosamine-1-phosphate transferase 77. Pfaffl MW, Horgan GW, Dempfle L: Relative expression software tool (REST (c)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 2002., 30: Authors’ contributions BLG carried out the macroarray experiment from design to hybridization; contributed to the sensitivity assays; initiated the qRT-PCR experiments; and helped to draft the manuscript. MG carried out most of the sensitivity assays of the yeast strains; helped in the analysis of the qRT-PCR data; deposited the array data at the GEO database; and contributed to draft the manuscript. AM participated in the initial conception of the approach; initiated the sensitivity assays; and performed the confocal microscopy experiments. LC completed the qRT-PCR experiments and carried out the corresponding analyses; and carried out the fluorescence microscopy and flow cytometry experiments. JFM conceived and coordinated the study; carried out the bioinformatic analysis of the macroarray data; and wrote the manuscript. All authors read and approved the final manuscript.

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