Methods and Results-Ninety-eight endothelial cell samples from 13 discrete coronary and noncoronary arterial

regions of varying susceptibilities to atherosclerosis were isolated from 76 normal swine. Transcript profiles were analyzed to determine the steady-state in vivo endothelial phenotypes. An unsupervised systems selleck biology approach using weighted gene coexpression networks showed highly correlated endothelial genes. Connectivity network analysis identified 19 gene modules, 12 of which showed significant association with circulatory bed classification. Differential expression of 1300 genes between coronary and noncoronary artery endothelium suggested distinct coronary endothelial FK228 phenotypes, with highest significance expressed in gene modules enriched for biological functions related to endoplasmic reticulum (ER) stress and unfolded protein binding, regulation

of transcription and translation, and redox homeostasis. Furthermore, within coronary arteries, comparison of endothelial transcript profiles of susceptible proximal regions to protected distal regions suggested the presence of ER stress conditions in susceptible sites. Accumulation of reactive oxygen species throughout coronary endothelium was greater than in noncoronary endothelium consistent with coronary artery ER stress and lower endothelial expression AZD1480 mw of antioxidant genes in coronary arteries.

Conclusions-Gene

connectivity analyses discriminated between coronary and noncoronary endothelial transcript profiles and identified differential transcript levels associated with increased ER and oxidative stress in coronary arteries consistent with enhanced susceptibility to atherosclerosis. (Circ Cardiovasc Genet. 2011;4:243-252.)”
“Patients with Crohn’s disease (CD) frequently require cross-sectional imaging. Magnetic resonance enterography (MRE) is an accurate tool for assessment of bowel disease and of various complications of CD. The lack of non-ionizing radiation exposure is an important advantage of this imaging modality. Familiarity with common and pathognomonic imaging features of CD is essential for every clinician that is involved in inflammatory bowel disease (IBD) patients care.

This review is aimed to describe the indications for performing MRE in CD, essentials of MRE techniques and typical radiological findings in patients with CD to aid the IBD doctor in daily practice. (C) 2012 European Crohn’s and Colitis Organisation. Published by Elsevier B.V. All rights reserved.”
“We present the synthesis and magnetic properties of multifunctional CoPtAu alloy nanoparticles of three compositions, Co(0.33)Pt(0.33)Au(0.33), Co(0.2)Pt(0.2)Au(0.6), and Co(0.4)Pt(0.4)Au(0.2), by a modified polyol process.