majuscula 3L genome; check details annotations in progress) both yielded a number PND-1186 solubility dmso of hypothetical protein matches in other cyanobacteria including Anabaena variabilis, Microcoleus chthonoplastes, Nostoc punctiforme, and Trichodesmium erythraeum (JHB protein BLAST hits in Table 2; see below). Interestingly, both proteins also matched (although significantly better for 7968) with the protein RcaD, an activator protein from the cyanobacterium Calothrix (= Fremyella diplosiphon or Tolypothrix) known to regulate complementary chromatic adaptation [32–35]. Complementary chromatic adaptation (CCA) is a phenomenon exhibited by many cyanobacteria in response to changes in light wavelength and intensity. CCA allows cyanobacteria

to alter pigment levels so as to optimize their capacity KPT-8602 nmr for photosynthesis, and usually involves variation between green and red phenotypes [36]. RcaD is a

protein that binds to the promoter for phycocyanin 2 (cpc2) and alters the expression of several red light operons in the acclimation phase of CCA [34, 35]. Another protein, RcaG, is located downstream of RcaD and has been identified as a putative ATPase. RcaG may facilitate binding of RcaD to DNA, and could require phosphorylation to complete this task [34]. Bioinformatic analysis of the L. majuscula 3L genome revealed that the proteins immediately downstream of 5335 and 7968 both resulted in BLAST hits with RcaG, although as with RcaD, the protein neighboring 7968 (7969) had much stronger sequence identity than the

neighboring protein to 5335 (5336). Table 2 BLAST results with Lyngbya majuscula JHB proteins 5335 and 7968. 5335 (279 aa)             Best BLAST hit BLAST organism Size (aa) identity similarity e value accession # hypothetical protein Nostoc punctiforme PCC 73102 217 56 70 8.00E-62 YP_001867255 hypothetical protein Microcoleus chthonoplastes PCC 7420 245 56 71 2.00E-59 ZP_05025825 hypothetical protein all4300 Nostoc sp. PCC 7120 227 49 68 4.00E-54 NP_488340 hypothetical protein Anabaena variabilis ATCC 29413 221 49 65 1.00E-51 YP_321771 hypothetical protein Lyngbya sp. PCC 8106 224 47 64 9.00E-47 ZP_01623947 hypothetical protein Lyngbya sp. PCC 8106 156 33 56 2.00E-11 ZP_01621638 hypothetical protein Nodularia Calpain spumigena CCY9414 100 41 61 3.00E-11 ZP_01628571 hypothetical protein Arthrospira maxima CS-328 131 32 60 1.00E-08 ZP_03271683 RcaD protein Tolypothrix sp. PCC 7601 285 22 48 0.2 CAC39267 7968 (304 aa)             Best BLAST hit BLAST organism Size (aa) identity similarity e value accession # hypothetical protein Cyanothece sp. PCC 7424 274 49 69 2.00E-68 YP_002380360 RcaD protein Tolypothrix sp. PCC 7601 285 43 63 3.00E-54 CAC39267 hypothetical protein Trichodesmium erythraeum IMS101 272 40 59 3.00E-52 YP_720119 hypothetical protein Nodularia spumigena CCY9414 280 44 62 1.00E-50 ZP_01631082 hypothetical protein Microcoleus chthonoplastes PCC 7420 287 41 62 7.00E-50 ZP_05025219 hypothetical protein Synechococcus sp. PCC 7335 199 33 57 3.