The Selected Fre-quencies Mode was used for analys with the interval selection set to continuous and the number of measure-ments set to . Measurements were to bepleted within 0 minutes of participants being supine. Clinical Pitting A pitting test was performed on each ankle during the water displacement volumetry sitting period. Par-ticipants were seated with both feet extend with the ankles being assessed in a neutral dorsi xion  ITMN-191 position. The assessor pressed and held his or her index ger over the bony prominence cm proximal to the mid-point of the medial malleolus of the ankle for seconds and graded the presence and severity of edema . Scores were converted to numeric values as follows: no pitting ; trace ;.. and . Ankle Circumference Ankle circumference was measured during the sit-ting period of water displacement volumetry.

A ten-sion-controlled measuring tape was used to minimize measurement error due to  differences in the amount of tension applied. After marking each ankle clined on the Dihydroquercetin examination table in a climate-controlled with a semipermanent marker at cm proximal to room in a fully supine position with arms extended at the sides. Four electrod each measuring the midpoint of the medial malleolu circumference of each ankle was measured were placed on the leg. A detection elec-twice while the participant was seated. March Clinical Therapeutics Water Displacement Volumetry After lying in the supine position required for bio-impedance measuremen participants were accli-mated to the sitting position prior to initiation of water displacement volumetry measurements. After sitting in a chair for 0 minut the volume of each foot/ankle was measur in duplica using  dyphylline 479-18-5 amercially available foot volumeter . The volumet a clear acrylic were at the -sided level. Mean dif-rectangular box measuring 3 in in 6 in with a ferences from placebo are provided. spout at the top of one of the short sid was led with lukewarm water until water rushed out of the spout. Once the water level was stab the participant placed foot in the volumeter and the displaced water was col-lected and weighed.

The weight of water displaced in grams equaled the volume of the foot/ankle in milliliters or cubic centimeters. Patient-Perceived Edema Questionnaire The Patient-Perceived Edema questionnaire was self-administered and included questions about the number of days  buy FK-506 between visits on which the participant experienced swollen ankl the degree of swelli and the limitations in normal activity caused by ankle swelling.

Statistical Analyses The analysis population consisted of all participants Clinical pitting was assessed for both ankles of each participant and counted separately. A Cochran-Man-tel-Haenszel statistic was calcu-lated to test whether the raw mean scores differed. The statistical test was conducted on the change from base-line in pitting score. No adjustment for multiplicity was necessary for the primary hypothesis because there was primary end point assessed at primary time point . Howev to facilitate clear con-clusions across all time points and preserve type I error at nominal for the set of time point  anatomy interpretation of the treatment-parison P values was based on a step-down procedure across the time points . It was calculated that for any individual.