There was no treatment, the wound Irinotecan Topoisomerase inhibitor heals very slowly with 50% of them recovered to 28 days after the formation of the skin wound. Treatment with accelerated wound healing rhaFGF: It was almost completely healed ndig at 21 and 25 days after treatment for pegylated and native rhaFGF group. Compared with treatment with the native rhaFGF, carried weight RhaFGF pegylated effective therapy, a significant importance, cure for 7 days after treatment. Furthermore, the healing of diabetic wounds was slightly shortened in rats administered with pegylated rhaFGF under the same molar concentration of native rhaFGF. Proliferation, remodeling and expansion: In general, each skin rest for three, and yet analyzed overlapping, phases. The histopathological examination with HE-F Staining showed that wounds treated rhaFGF PEG showed progress in three phases. 6A shows that in both groups of PEG and rhaFGF rhaFGF, there was a significant increase in fibroblasts at day 7, in particular in the pegylated rhaFGF group which has almost the same amount of fibroblasts compared to the control group. But in the diabetic group there was a big e number of inflammatory cells and fibroblasts little. On day 14 in the control group, there was still a big number of e fibroblasts, inflammatory cells are gone, and mature vascular E were taking into account the size Differ E. In both native and PEG groups were rhaFGF rhaFGF leave it only a few inflammatory cells, but there was also a big e number of fibroblasts and mature blood vessels E were distinguished in the light of the size E. W While in the diabetic group, there were many inflammatory cells, and a few fibroblasts. On the other hand, the formation of new blood vessels S was necessary to maintain the newly formed granulation tissue upright.
Grace on the histological examination was Gefitinib 184475-35-2 observed that the proliferation of blood vessels was observed S in groups rhaFGF PEG faster than the diabetic group. Since the formation of collagen is a critical step in wound healing, found We rbt the skin tissue with Masson trichrome what can highlight collagen remodeling and maturation. In the groups treated for 7 and 14 days, showed Masson Trichromf Staining that the more mature collagen developed in the wounds treated rhaFGF PEGylated treated with such native rhaFGF. The results of the Masson-Trichrome stain support , quantitative analysis of the essential marker of the expression of TGF b1 fibrosis was examined by Western blotting. TGF B1 can accelerate the differentiation of endothelial cells, there may be also f Rdern and proliferation of the fibroblast cell matrix. Figure 7 was shown that PEG treatment rhaFGF h Here induces expression compared with TGF b1 rhaFGF native andsimilar than in non-diabetic rats treated with saline Solution on day 7 after initiation of therapy. As a marker of cell proliferation, 24 PCNA expression by Western blotting was analyzed. It was shown that the expression of PCNA in the skin of the wounded diabetic rats treated with PEG rhaFGF h Chsten was at day 7 in the treated groups. Immunohistochemical F Best dyeings Saturated the results of the Western blot test, the PCNA-positive cells in the h Chsten showed injured sk.