the cooking process did not significantly af


the cooking process did not significantly affect the BG content for either cultivation method, and this was reported previously ( Rungapamestry et al., 2006 and Verkerk et al., 2009). Among the analyzed vegetables, watercress behaved differently. No significant difference in benzylglucosinolate content was observed between the organically and conventionally cultivated plants. Among the other analyzed Brassicaceaes, organic collard greens had the highest BG content ( Fig. 2). In conclusion, the organic cultivation practice led to increased concentrations of total glucosinolates and benzylglucosinolate in most of the vegetables. These differences were more apparent when the compounds were isolated and separated using HPLC high resolution liquid chromatography. The acidified methanol extraction of broccoli tissues resulted in significantly higher levels of GLs, which Cabozantinib in vivo differentiated the two modes of cultivation. This difference was supported by the chromatographic analysis of benzylglucosinolate. The tissue extract analysis without the addition of TFA revealed the same concentration profile, but the concentrations of compounds were much lower. Among the evaluated Brassicaceaes, watercress exhibited a different profile for benzylglucosinolate and GL concentration;

significantly higher concentrations of the compounds were IWR-1 cell line observed in conventionally cultivated watercress. These results suggest that watercress cultivated conventionally is Adenosine triphosphate more efficient at sulfur absorption. The highest levels of glucosinolates and benzylglucosinolate were found in Brassica cabbage and broccoli. Furthermore, cooking significantly decreased the GL content of vegetables, but the more accurate HPLC analysis showed that the benzylglucosinolate profile was unaffected. Thus, we believe that these types of plants, if cultivated organically, may become promising sources of secondary metabolites and may reveal gene targets that could confer resistance against phytopathogenic pests and diseases of agro-economic importance; this would contribute to environmental sustainability

without the use of radical agricultural production systems. The authors thank FAPESPand CNPq for supporting this work. We also thank Beatriz Rosana Cordenunsi and Eduardo Purgatto (Laboratory of Food Science Faculty of Pharmaceutical Science-University of São Paulo – SP/Brazil) for assistance with HPLC analyses. “
“A significant proportion of patients undergoing renal replacement therapy (RRT) with hemodialysis (HD) or peritoneal dialysis present a microinflammatory state, which is clinically detected by increased levels of C-reactive protein (CRP) and other inflammatory markers, mainly interleukin 1 and interleukin 6 [1] and [2]. This proinflammatory state is predictive of higher mortality levels and is associated with the malnutrition, inflammation, and atherosclerosis syndrome [3] and other factors, including the dialysis treatment itself [4], [5] and [6].

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