Ethyl acetate fraction of the ethanolic extract of L. lanata was prepared and the percentage yield was found to be 0.248%w/w. From the HPTLC studies it was observed that, there were 3 flavonoids in the LLEA fraction and was not containing the standard flavonoids, quercetin, rutin and kaempferol. Among the identified flavonoids, flavonoid 1 was found at 0.03 Rf value with 1045.0 plot area and 6.55% relative percentage. Flavonoid 2 was found at 0.48 Rf value Dactolisib ic50 with 1292.1 plot area and 8.10% relative percentage.

Flavonoid 3 was found at 0.93 Rf value with 822.1 plot area and 5.15% relative percentage. The Rf value of standard flavonoids, quercetin, rutin and kaempferol was found to be 0.20, 0.01 and 0.36 respectively. For antiepileptic activity the results of durations of hind limb extension, immobility times in forced swim test and malondialdehyde content in extracted brains of animals were given in Table 5. Most of the recent investigations have proved the free radical scavenging activity of the phytoconstituents especially flavonoids. Flavonoids are recently given considerable scientific and therapeutic interest and they offer protection from free radicals damage.20 Phytoconstituents like glycosides from Leucas genus were found to have free radical scavenging activity. 21 In our present investigation after

phytochemical screening, the extract was found to contain considerable amounts of flavonoids (64.412 ± 8.44 mgGAE/g) and phenolic compounds (63.723 ± 8.01 mgRE/g). Studies on free radical scavenging activity revealed that, the IC50 values of the extract were found to be almost equal to the IC50 values of quercetin except for 1, 1-diphenyl-2-picryl GSK1120212 solubility dmso hydrazyl radical scavenging. The preliminary studies indicated the presence of flavonoids Sodium butyrate and with the positive values from free radical scavenging activity, the presence of flavonoids was almost confirmed. The same was further confirmed from the HPTLC studies. There were 3 unknown flavonoids revealed from HPTLC run of ethyl acetate fraction

of L. lanata. Univalent reduction of oxygen produces free radicals and these are found to produce damage to blood vessels and parenchyma of the brain. Especially in seizures, these free radicals were involved in causation of lipid peroxidation, brain edema, dysfunction including coma and death.22 Even in current scenario, epilepsy continues to be a neurological disorder awaiting the use of safer drugs. For the antiepileptic studies in mice, pentylenetetrazole was used to induce seizures in mice. Pentylenetetrazole induced seizure activity mimics the increased oxidative stress in brain by altering membrane phospholipid metabolism and ultimately resulting in the release of free radicals.19 To assess the seizure activity, duration of hind limb extension was measured. In control group there might be damage in brain due to the free radicals produced by pentylenetetrazole and hence the duration of hind limb extension was more.