Densitometry (DPX) was performed at femoral neck. The 10-year risk of fracture was assessed according to the British model of FRAX calculator.\n\nResults: The study group was divided into two, depending on the history of low-energy fractures. Previous osteoporotic fractures were confirmed in 128 patients. In this group, the mean bone mineral density (BM
D) values (0.717 g/cm(2)) were lower than in the group without fracture history (0.735 g/cm(2)). In 33.3% of patients aged 50-59 years and 17% of women aged 60-79 who required medical treatment for their clinical status (previous selleck kinase inhibitor fracture), the FRAX value did not meet the criterion of pharmacotherapy administration. Considering BMD in the calculation of FRAX produced an even higher underestimation of the fracture risk. Of women aged 40-49, 25% were qualified for pharmacotherapy of osteoporosis. In that particular age category, BMD did not affect the FRAX value. BMD measurement had a higher discriminatory value among patients aged 50-79, increasing the number of patients requiring therapy by more than 50%.\n\nConclusions:\n\n1. The FRAX calculator does not always consider the history of low-energy fractures as a criterion sufficient for therapy implementation.\n\n2. Designing a FRAX calculator specifically for the
Polish population would be advisable. (Pol J Endocrinol 2011; 62 (1): 30-36)”
“Methanogenic archaea possess unusual seryl-tRNA synthetases (SerRS), evolutionarily MLN2238 manufacturer distinct from the SerRSs found
in other archaea, eucaryotes and bacteria. Our recent X-ray structural analysis of Methanosarcina barkeri SerRS revealed an idiosyncratic N-terminal domain and catalytic zinc ion in the active site. To shed further light on substrate discrimination by methanogenic-type SerRS, we set up to explore in vivo the interaction of methanogenic-type SerRSs with their cognate tRNAs in Escherichia coli or Saccharomyces cerevisiae. The expression of various methanogenic-type SerRSs was toxic for E. coli, resulting in the synthesis of erroneous proteins, as revealed by beta-galactosidase stability assay. Although SerRSs from methanogenic archaea recognize tRNAs(Ser) from all three domains of life in vitro, the toxicity Selleck PCI-34051 presumably precluded the complementation of endogenous SerRS function in both, E. coli and S. cerevisiae. However, despite the observed toxicity, coexpression of methanogenic-type SerRS with its cognate tRNA suppressed bacterial amber mutation.”
“It has been widely accepted that the gas diffusion through a glassy polymer can be related to the fractional free volume of the polymer through the Doolittle relation D=A exp(-B/f) where f is the fractional free volume and A and B are constants. As the free volume increases and pores become connected and bi-continuous the Doolittle relation does not adequately model the experimental data.