BPA was more effective spinogenesis, incubation 5 α reductase of state to the following tests were used, unless otherwise indicated. To the signaling induced increase inthe total pathwayofBPA S Ulendichte study, antagonists and inhibitors were used more. Application of ICI, an antagonist of the ERA / Erb, not suppress increased Hte density of the vertebra column Of 10 nM BPA. Moreover, the application of OH-Tam, an antagonist both ERRC and ERA / Erb, completely Abolished ndig, improving the density of the vertebra Molecules of the BPA. Application of MK 801, an NMDA receptor antagonist completely, YOUR BIDDING improving the density withdrawn by the cyclone Molecules through the BPA. Application of CNQX, AMPA receptor antagonists, partially prevented the development of the BPA. Administration of nicardipine, an antagonist of L-type voltage Ca2 channel no inhibitory effect improving the density of the vertebra Molecules induced by 10 nM BPA. When Ca2 free ACSF was used to induce spinogenesis BPA completely Ndig was suppressed. Application of PD98059, an inhibitor of MAP kinase Erk completely, YOUR BIDDING inhibited the induced spinogenesis BPA. The leaching of BPA induced for a further 2 h after 2 h abolished the BPA BPA Erh Increase the spinogenesis, which means that the effect of BPA was reversible. It should be noted that the inhibitors alone did not significantly affect the overall density of the vertebra Molecules to experimental error, indicating that the inhibitory effect shall not be observed by simple blocker  effects of the poison. 3.1.2. Analysis vertebra Molecules IkB Pathway head diameter Morphological Ver Changes in diameter of the cyclone Column head by a 2 hr treatment with 10 nM BPA were induced evaluated. Most spines had a clear head, so we have statistically analyzed these spines pfennigs with different K. We classified spines into three categories according to their diameter of the head, ie head spines small, medium spiny head, big head and he backs. The categorization into three subclasses allowed distinguish different subpopulations reactions vertebra Column may need during the application of BPA, receptor antagonists and kinase inhibitors. Head spines small, medium, large and differ from the density of AMPA receptors, therefore, k can these three types of spines have different physiological functions. AMPA receptor density in the vortex Molecules positively correlated with the size E of the post-synaptic. We performed statistical analysis using three sub-classes. Contr into slices From the density of the vertebra Column was 0.14 spines / thorns LM, with small spines, 0.40 / LM middle head thorns, spines and 0.45 / LM for large lead S head spines. W During treatment with BPA, the density of thorns head east markedly increased to 0.60 spikes / LM Ht, w While the density of the small head, big head spines em was not significant VER Changed. The coapplication of OH significantly suppressed with Tam BPA BPA-induced increase in the density of spines Middle Head to 0.35 spines / LM. No significant Change was the BPA-induced increase in the density of the three subclasses of spines induced by the administration with co HERE. 3.2. The color distribution of the hippocampal NDRC ERRC Acadesine was examined by immunostaining Staining of hippocampal slices with antiques Rpern against the ERRC. A strong immunostaining Was rnerzellen staining of pyramidal cells and K Observed in CA1 and CA3 DG. No F Staining was observedThe raising the BPA 10 nM induced a rapid increase in.