05), while other inhibitors (ERK inhibitor, JNK inhibitor and PKA inhibitor) had no effect on the induction of smad7 by exogenous TGF-β3 stimulation (P > 0.05). 6) In basal condition, exogenous TGF-β1 also increased smad7 mRNA expression in HSC (1.5-fold higher than control, P < 0.05), but this induction is lower AG-14699 than it by exogenous TGF-β3. Additionally, the inhibition and over-expression of CREB-1 had no effect on exogenous TGF-β1-induced smad7 expression in HSC (P > 0.05). Conclusion: 1) TGF-β3 increases smad7 expression in HSC. 2) smad3 is an important transcriptional regulator for smad7. 3) CREB-1 is critical for TGF-β3-induced samd7 in HSC. 4) TGF-β3 activates CREB-1
by p38 in HSC. Taken together, TGF-β3 might activate both smad3 and CREB-1, and CREB-1 is an important co-transcriptional factor which enhances the binding of smad3 with DNA, caused a continuous
induction of smad7 in HSC, and CREB-1 might contribute to resist liver fibrosis. Key Word(s): 1. Liver fibrosis; 2. CREB-1; 3. TGF-β3; 4. smad7; Presenting Author: YANHUA SHEN Additional Authors: HAIXING JIANG Corresponding Author: HAIXING JIANG Affiliations: 1st Affiliated hospital of Guangxi medical university Objective: To investigate the effect of activated hepatocyte growth factor (HGF) on hepatic stellate cells (HSCs) apoptosis and the regulation of Rho pathway. Methods: HSCs were divided into the following groups: check details ① the blank control group: HSCs were cultured alone; ② the control group: a. HSCs were cultured with exogenous HGF (50 ng/ml), b. HSCs were cultured with exogenous HGFA (70 ng/ml); ③ the experimental group: HSCs were co-cultured with exogenous HGF and HGFA; ④ HGF inhibitor groups: HSCs were incubated with c-met (500 ng/ml) blockers for 6 hours, and then with exogenous HGF and HGFA; ⑤ Rho pathway inhibitor groups: HSCs were cultured with Y-27632
(10 ng/ml), and then with exogenous HGF and HGFA. The activation of HSC was determined by analysis of alpha smooth muscle actin (α-SMA) expression. The best intervention concentration of Y – 27632 was detected by MTT assay; MCE公司 HSCs apoptosis was tested by Flow Cytometry; the expression of HGF alpha chain was determined by Immunofluorescence; RohA mRNA levels were evaluated by PCR. Protein expressions were evaluated by immunohistochemical staining and Western blot analysis. Results: ① Y-27632 at 10 μ mol/L caused obviously HSCs inhibition (P < 0.01) compared with other concentration groups. ② The expression of the HGF-α chain showed time-dependent increased manner (P < 0.01). However, there was no statistic difference (P > 0.05) in blank control group and control group. ③ The apoptosis rate increased over time (24 h, 48 h, 72 h) (P < 0.01). The experimental group caused the highest levels (P < 0.01). ④ The expression of RhoA mRNA in experimental group decreased over time (P < 0.01) and caused the lowest levels compared with othergroups (P < 0.01).