L=lumen of gland; G=prostate

gland Figure 3 Microscopic sections (Haematoxylin & Eosin staining, Mag. x100) of the prostate of rats treated with 25 mg/100 g body weight of Momordica charantia seed extract for 8 weeks (a) and the extract for eight weeks followed by physiological saline for eight weeks after withdrawal of the extract (b). Stains: Haematoxylin & Eosin. Mag. x100; L=lumen of gland; G=prostate gland Figure 4 Microscopic sections (Haematoxylin & Eosin staining, Inhibitors,research,lifescience,medical Mag. x100) of the prostate of rats treated with 50 mg/100 g of Momordica charantia seed extract for 8 weeks (a) and the extract for eight weeks followed by physiological saline for eight weeks after withdrawal of the extract (b). L=lumen of gland; G=prostate gland Figure 5 Microscopic Inhibitors,research,lifescience,medical sections (Haematoxylin & Eosin staining, Mag. x100) of the seminiferous tubules of control rats (receiving normal saline) sacrificed at the end of eight weeks (a) and 16 weeks (b). L=lumen of seminiferous tubule; SE=seminiferous epithelium; I=testicular interstitium seminiferous epithelium; I=testicular interstitium Figure 6 Microscopic sections (Haematoxylin & Eosin staining, Mag. x100) of the seminiferous tubules rats treated with 15 mg/100 g of Momordica charantia seed extract for eight weeks (a) and the extract for eight weeks followed by physiological saline for eight weeks after withdrawal of the extract (b). L=lumen

of seminiferous tubule; Inhibitors,research,lifescience,medical SE=seminiferous epithelium; I=testicular interstitium Figure

7 Microscopic sections (Haematoxylin & Eosin staining, Mag. x100) of the seminiferous tubules rats treated with 25 mg/100 g of Momordica charantia seed extract for eight weeks (a) and the extract for eight weeks followed by physiological saline for eight weeks after withdrawal Inhibitors,research,lifescience,medical of the extract Inhibitors,research,lifescience,medical (b). L=lumen of seminiferous tubule; SE=seminiferous epithelium; I=testicular interstitium Figure 8 Cross-section of the seminiferous tubules of rat treated with 50 mg/100 g of Momordica charantia seed extract for 8 weeks (a) and treatment with physiological saline for another 8 weeks after withdrawal of extract (b). Stains: Haematoxylin & Eosin. Mag. x100; L=lumen of seminiferous tubule; SE=seminiferous epithelium; I=testicular interstitium Discussion The histology of the testes and prostate showed dose-dependent degenerative changes as a result of the treatment Dipeptidyl peptidase with graded doses of methanolic extract of MC seed. On the seminiferous tubular epithelium, the changes ranged from a decrease in the Fulvestrant in vitro number of germ cells to a reduction in the sizes of interstitial connective tissue/Leydig and Sertoli cells. There is also associated widening of the seminiferous tubules as well as decrease in percentage spermatozoa populating the tubular lumen. The prostate showed an increased luminal secretions and dilatation resulting in the crowding of the glands. These observations suggest degenerative changes in the prostate and testes.