, 1953; Fossati and Prencipe, 1982; Falholt et al., 1973 [20], [21] and [22] respectively. The phospholipids estimation was done by the method of Zilversmit and Davis, 1950 [23] Lipid peroxidation in plasma, liver and kidney was estimated spectrophotometrically by measuring thiobarbituric acid reactive substances and lipid hydroperoxides by the method of Niehius and Samuelson, 1968; Jiang et al., 1992 [24] and [25] respectively. GSK3235025 concentration Superoxide dismutase activity was determined by the method of Kakkar et al., 1984 [26]. The activity of catalase
was determined by the method of Sinha et al., 1972 [27]. Glutathione peroxidase activity was estimated by the method of Rotruck et al., 1973 [28]. Glutathione S-transferase activity was determined by the method of Habig et al., 1974 [29]. Vitamin C concentration was measured as previously reported check details Omaye et al., 1979 [30]. Vitamin E (α-tocopherol) was estimated by the method of Desai et al., 1984 [31]. Reduced glutathione was determined by the method of Ellman et al., 1959
[32]. The liver and kidney sample fixed for 48 hr in 10% formalin were dehydrated by passing successfully in different mixture of ethyl alcohol–water, cleaned in xylene and embedded in paraffin. Sections of liver and kidney (5–6 μm thick) were prepared and then stained with hematoxylin and eosin dye, which mounted in neutral DPX medium for microscopic observations. Values are given as means ± S.D for six rats in each group. Data were analyzed by one-way analysis of variance followed by Duncan’s Multiple Range Test (DMRT) using SPSS version 13 (SPSS, Chicago,
IL). The limit of statistical significance was set at (P < 0.05) and the values sharing a common superscript did not differ significantly. Table 1 depicts the levels of serum hepatic markers in control and experimental rats. In Fe treated rats, the activities of serum hepato-specific enzymes such as aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, gamma glutamyl transferase and the levels of bilirubin were significantly increased (P < 0.05). Administration of hesperidin significantly reversed these changes in a dose dependent manner. Table 2 presents the Cyclin-dependent kinase 3 levels of renal functional markers in control and experimental rats. In Fe treated rats, the activities of renal functional markers such as urea, creatinine, creatinine clearance and haemoglobin were significantly increased (P < 0.05). Administration of hesperidin significantly (P < 0.05) reversed these changes in a dose dependent manner. Our results indicate that hesperidin at a dose of 80 mg/kg body weight was more effective than other doses (20 and 40 mg/kg body weight). Hence, hesperidin 80 mg/kg body weight was used for further biochemical studies. The concentration of iron has been depicted in Fig. 2. Fe administration to normal rats resulted in a significant (P < 0.