We proposed

an integrated microfluidic chip for direct an

We proposed

an integrated microfluidic chip for direct and rapid trapping WBCs from whole blood. The microfluidic chip consists of two basic functional units: a winding channel to mix and arrays of two-layer trapping structures to trap WBCs. Red blood cells (RBCs) were eliminated through moving the winding channel and then WBCs were trapped by the arrays of trapping structures. We fabricated the PDMS (polydimethylsiloxane) ARS-1620 molecular weight chip using soft lithography and determined the critical flow velocities of tartrazine and brilliant blue water mixing and whole blood and red blood cell lysis buffer mixing in the winding channel. They are 0.25 mu l/min and 0.05 mu l/min, respectively. The critical flow velocity of the whole blood and red blood cell lysis buffer is lower due to larger volume of the RBCs and higher kinematic viscosity of the whole blood. The time taken for complete lysis of whole blood was about 85 s under the flow velocity 0.05 mu l/min. The RBCs were lysed completely by mixing and the WBCs were trapped by the trapping structures. The chip trapped about 2.0 x 10(3) from 3.3 x 10(3) WBCs. (C) 2013 AIP Publishing LLC.”
“Background: The purpose of this retrospective study was to analyze the functional and radiographic results of cementless, modular total hip arthroplasty combined with subtrochanteric osteotomy for the

treatment of patients who had had Crowe Group-IV developmental dysplasia of the hip as a child.

Methods: Twenty-five consecutive patients

(thirty-three hips) who had previously had Crowe Group-IV selleck screening library developmental dysplasia of the hip were treated with a modular cementless prosthesis at a mean age of sixty years. The mean follow-up period was eight years (range, five to eleven years). The acetabular cup was placed in the position of the anatomical hip center in every patient. Subtrochanteric femoral shortening osteotomy was performed with use of a step-, cut design.

Results: The mean Merle d’Aubigne and Postel hip score improved from 9 to 16 points (out of a maximum of 18 points). The mean limb-length discrepancy in seventeen patients with unilateral involvement was reduced from 5.1 cm (range, 3.7 to 6.5 cm) to 2.8 CHIR98014 order cm (range, 1.4 to 4.6 cm). Two,patients had a positive Trendelenburg sign, and three had a slight limp at the time of the latest follow-up. No cases of nonunion or nerve palsy were encountered. Postoperative dislocations occurred in two hips. One hip showed progressive radiolucent lines around the proximal femoral sleeve within two years after the surgery, and this was followed by progressive stem subsidence. Only one femoral stem was revised.

Conclusions: Cementless, modular total hip arthroplasty combined with subtrochanteric osteotomy for the treatment of patients with prior Crowe Group-IV developmental dysplasia of the hip resulted in satisfactory outcomes.

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