None of the patients had complications. Conclusion: The majority of very elderly FOBT-positive patients without visible blood in the stool had no abnormalities or low-grade adenoma when the polyp was small, showing that advanced colon cancer was relatively rare. Considering their advanced age, colon polyps are unlikely to Sirolimus cost progress rapidly to cancer in very elderly patients. Thus, instead of offering colonoscopy to all very elderly FOBT-positive patients, non-invasive

abdominal computed tomography may be useful to select those with suspected advanced colon cancer for further examination by colonoscopy. Key Word(s): 1. very elderly patient; 2. FOBT-positive; 3. colonoscopy Presenting Author: HONGJIE ZHANG Additional Authors: XIUFANG CUI Corresponding Author: HONGJIE ZHANG Affiliations: Jiangsu People Hospital Objective: The present study was designed to investigate the effect of GLP-1 analogue exendin-4 on visceral hypersensitivity in rat model, and its possible regulation on SERT expression and 5-HT reuptake. Methods: Neonatal male Sprague-Dawley rats received intra-colonic injection of 0.5% acetic acid. Visceral sensation was determined

by assessing Trichostatin A datasheet abdominal withdrawal reflex (AWR) and electromyography (EMG) activity. Exendin-4 with doses of (1, 5, and 10 μg/kg) was administered by intra-peritoneal injection. SERT expression was detected by quantitative PCR (qRT-PCR)

and Western blotting. SERT function was determined by tritiated 5-HT reuptake experiment in IEC-6 cells. Forskolin, protein kinase A (PKA) inhibitors (H89) or adenylyl cyclase inhibitor (SQ22536) was used to investigate the GLP-1/ cAMP/PKA signaling pathway. Results: Neonatal acetic acid (AA) Cyclin-dependent kinase 3 intra-colonic treatment presented hypersensitivity to CRD in adult rats compared with controls. High levels of 5-HT were detected in plasma and colonic tissues in AA-treated rats (P < 0.05). Stimulated with exendin-4 at 10 μg/kg could reduce visceral sensation. The expressions of SERT reduced in colon of the AA-treated rats, and increased after treatment with exendin-4. The expressions of SERT up-regulated and 5-HT reuptake function enhanced in IEC-6 cells after treatment with exendin-4 in dose- and time-dependently manner. The former effect was abolished by pre-treatment with exendin-9, SQ22536 and H89. Exendin-4 and forskolin increased PKA activity in IEC-6 cells. Conclusion: Exendin-4, a GLP-1 analogue, can attenuate hyperalgesia in rats with neonatal colon sensitivity by up-regulating SERT expression and 5-HT reuptake, and its effect may involve in cAMP/PKA signaling pathway. Key Word(s): 1. irritable bowel syndrome; 2. glucagon-like peptide-1; 3. serotonin transporter Presenting Author: MURDANI ABDULLAH Additional Authors: D. MAKMUN, U.MAIMUNAH, ARLES, KUSNANTO, S.MIRO, SUYATA, NENENG, MARCELLUS S.