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34. Sambrook J, Russell DW: Molecular Cloning; a laboratory manual. 3 Edition Cold Spring Harbor Laboratory Press., Cold Spring harbor, N. Y 2001. 35. Thompson JD, Higgins DG, Gibson TJ: CLUSTAL Apitolisib molecular weight W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids Res 1994, 22:4673–4680.CrossRefPubMed Authors’ contributions JH, TS, AT and IT were involved with cloning, sequencing and for analysis of the rRNA gene sequences from Campylobacter strains. JEM and BCM participated in its design and coordination, and review of the manuscript. MM participated in design of the study, collected strains, drafted the manuscript and reviewed

the manuscript. All authors read and approved the final version.”
“Background Helicobacter pylori colonizes about half of the human population and is associated with several gastrointestinal diseases, such as gastritis, peptic ulcer, and gastric cancer [1, 2]. The similar pattern of human and H. pylori geographic diversity and distribution suggests a co-evolution between bacteria and man, which can be used to understand human migrations [2]. The H. pylori distribution pattern follows the human migration roots, which suggests that the colonization of the human stomach occurred before modern man left East Africa [2–5]. Several H. pylori gene alleles present different prevalence rates among the world H. pylori population. This is the case for vacA that presents allelic diversity of the s-, m- and i-region [6, 7].